Cell
lines (primary cultures):
- human umbilical vein endothelial cells (A. Albini, F. Bussolino, M.G. Lampugnani, G. Camussi)
- bovine coronary venular endothelial cells (M. Ziche)
- bovine aortic endothelial cells (M. Presta)
- mouse brain capillary endothelial cells (F. Bussolino)
- Kaposi's sarcoma cells (A. Albini)
- human corneal fibroblasts (M. Ziche)
- human smooth muscle cells (F. Bussolino)
- rabbit aortic smooth muscle cells (M. Ziche)
- rat aortic smooth muscle cells (M. Ziche)
- human mesothelial, human kidney mesangial cells, tubular cells, and fibroblasts (G. Camussi)
Endothelial
cell lines (immortalized):
- adenovirus-immortalized mouse brain capillary endothelial cells (F. Bussolino)
- polyomavirus mT antigen-immortalized mouse endothelial cells from different anatomical districts (F. Bussolino)
- spontaneously-immortalized mouse aortic endothelial cells (M. Presta)
- spontaneously-immortalized mouse brain capillary endothelial cells (M. Presta)
- spontaneously-immortalized mouse heart capillary endothelial cells (M. Presta)
- benzopyrene-transformed fetal bovine aortic endothelial GM 7373 cells (M. Presta)
Cell
lines (stable transfectants):
- VE-cadherin (M.G. Lampugnani)
- PlGF-1, PLGF-2 (M. Persico)
- VEGF121, VEGF165 (M. Persico)
- bFGF, K-FGF (M. Presta)
- FGFR-1, FGFR-2, FGFR-3, FGFR-4 (M. Presta)
- dominant negative FGFR-1 (M. Presta)
cDNA
(probes):
- NO synthases (F. Bussolino)
- VE-cadherin (M.G. Lampugnani)
- beta-catenin (M.G. Lampugnani)
- plakoglobin (M.G. Lampugnani)
- protocadherin (M.G. Lampugnani)
- PlGF (M. Persico)
- VEGF (M. Persico)
- KDR/Flk-1 (M. Persico)
- FLT-1 (M. Persico)
- bFGF, K-FGF (M. Presta)
- FGFR-1, -2, -3, -4 (M. Presta)
- uPA, uPA receptor (M. Presta)
- syndecan-1 (M. Presta)
- perlecan (A. Albini)
Oligonucleotide
primers:
- synthesis of oligonucleotides (A. Albini)
- human bFGF (M. Presta)
- human and murine PlGF and VEGF (G. Persico)
- rat VEGF (G. Viglietto)
- human FLT-1 and KDR (G. Viglietto)
- p53 and BCL-2 (G. Viale)
cDNA
and genomic libraries:
- cDNA libraries from undifferentiated NTERA2/D1 (in lambda GT10), human placenta (in lambda GT11), human colon carcinoma (GEO) cells (in lambda ZapII), mouse brain (in lambda GT10), mouse hearth (in lambda ZapII) (M. Persico).
- Genomic libraries : human (in lambda FIX) and mouse (in EMBL3) (M. Persico)
Recombinant
proteins:
- human HGF (F. Bussolino)
- human HGF mutants (F. Bussolino)
- human bFGF (M. Presta)
- human bFGF mutants (M. Presta)
- human TNF-alpha (G. Camussi)
- human PlGF (M. Persico)
Antibodies:
- polyclonal anti-murine T lymphocyte (G. Camussi)
- monoclonal anti-L3/T4 (G. Camussi)
- monoclonal anti-Ly2 (G. Camussi)
- monoclonal anti-Mac1 (G. Camussi)
- polyclonal anti-von Willebrand Factor (G. Camussi, G. Viale)
- monoclonal anti-Factor VIII (G. Gasparini)
- polyclonal anti-beta-1, alpha-1, alpha-2, alpha-3, alpha-5, alpha-v integrins, affinity-purified (G. Camussi)
- monoclonal GOH3 anti-alpha-6 integrin (G. Camussi)
- anti-alpha-2, alpha-v integrins, neutralizing (G. Camussi)
- monoclonal anti-alpha-v/beta-3 integrin (G. Gasparini)
- monoclonal TEC-11 anti-endoglin (G. Gasparini)
- monoclonal E-9 (G. Gasparini)
- monoclonal anti-human VE-cadherin, three clones directed to different epitopes (M.G. Lampugnani)
- polyclonal anti-CD31 (G. Viale)
- monoclonal anti-CD31 (G. Gasparini)
- polyclonal anti-CD34 (G. Viale)
- monoclonal anti-human PECAM-1 (M.G. Lampugnani)
- rat monoclonal anti-murine PECAM-1 (M.G. Lampugnani)
- polyclonal anti-bFGF (M. Presta, G. Viale)
- polyclonal anti-bFGF, neutralizing (G. Camussi)
- polyclonal anti-PlGF, affinity purified (M. Persico)
- polyclonal anti-PDGF (G. Viale)
- polyclonal anti-TGF beta (G. Viale)
- polyclonal anti-IGF (G. Viale)
- monoclonal anti-VEGF (G. Gasparini)
- polyclonal anti-p53 (G. Viale)
- polyclonal anti-BCL-2 (G. Viale)
- polyclonal anti-Rb (G. Viale)
- polyclonal anti-proliferating cells (Ki67, MIB1, PCNA) (G. Viale)
Human
tumor biopsies:
- 700 normal and tumor lung, paraffin-embedded and frozen (G. Fontanini)
- 100 lung carcinomas, frozen (G. Viale)
- 50 normal and tumor kidney, paraffin-embedded and frozen (G. Fontanini)
- 200 breast carcinomas, frozen (G. Gasparini)
- 500 normal and tumor breast, frozen (G. Viale)
- 30 breast carcinomas (G. Viglietto)
- 20 colon carcinomas (G. Viglietto)
- 30 colo-rectal carcinomas, frozen (G. Viale)
- 30 gastric tumors, frozen (G. Viale)
- 60 ovary carcinomas (G. Viglietto)
- 20 testis carcinomas (G. Viglietto)
- Different normal and tumor tissues, paraffin-embedded and/or frozen (L. Ruco)
- 300 reactive or metastatic lymphonodes, frozen (G. Viale)
Angiogenesis
assays (in vivo):
- Matrigel pellet s.c. in mice, blood vessel quantitation and mRNA detection (A. Albini;
- Rabbit cornea (M. Ziche)
F. Bussolino)
Angiogenesis
related-assays (in vitro):
- Morphogenesis on Matrigel (A. Albini, F. Bussolino, G. Camussi, M. Presta)
- Endothelial cell tube formation on 3-D collagen gel (M. Presta)
- Sprout formation within 3-D fibrin gel (M. Presta)
- Chemotaxis and chemoinvasion (A. Albini, F. Bussolino)
- Endothelial cell proliferation (A. Albini, F. Bussolino, M. Presta, M. Ziche)
- Plasminogen activator production (M. Presta)
- Collagenase production (A. Albini)
Endothelium-related
assays:
- NO synthase (F. Bussolino, M. Ziche)
- PAF production (F. Bussolino, G. Camussi)
- Vasopermeability assay in vitro and in vivo (F. Bussolino, M.G. Lampugnani)
- Calcium influx, intracellular pH, tyrosine and serine/threonine kinases in endothelial cells
- Leukocyte and tumor cell adhesion/transmigration to endothelium (M.G. Lampugnani)
- Pharmacologic modulation of vasodilation in rat or rabbit aorta and mesenteric artery
(F. Bussolino)
(M. Ziche)
Tumor
biopsy studies:
- immunohistochemistry (G. Fontanini, G. Gasparini, L. Ruco, G. Viale)
- blood vessel count (G. Fontanini, G. Gasparini, L. Ruco, G. Viale)
- in situ hybridization (A. Albini, L. Ruco, G. Viale)
Miscellanea: